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mouse anti c fos  (Proteintech)


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    Structured Review

    Proteintech mouse anti c fos
    Mouse Anti C Fos, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+fos/FOS+Antibody/pm42864341-540-13-15
    Average 95 stars, based on 124 article reviews
    mouse anti c fos - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    other:

    Article Title: A novel METTL3 inhibitor Nimbolide ameliorates osteoporosis via orchestrating osteoclastogenesis in an m6A-dependent manner
    Article Snippet: Please cite this article as: Chenhui Cai , Zaoqing Zhang , Xu Zhao , Chuan Yang , Xianming Huang , Chao Tang , Hao Qiu , Sizhen Yang , Ying Zhang , Xu Hu , Rui Zuo , Chao Zhang , Yueqi Chen , Tongwei Chu , A novel METTL3 inhibitor Nimbolide ameliorates osteoporosis via orchestrating osteoclastogenesis in an m6A-dependent manner, Phytomedicine (2026), doi: https://doi.org/10.1016/j.phymed.2026.158048

    Article Title: Uncovering cantharidin’s mechanism for cholangiocarcinoma treatment using patient-derived tumor organoids
    Article Snippet: c-Fos , Proteintech , Cat# 66590-1-Ig; RRID: AB_2881950.

    Immunofluorescence:

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress-Induced Depression in Male Mice.
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5-μm-thick transverse slices. .. For immunofluorescence analysis, the 30-μm-thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA-1 (GB12105, Servicebio), CaMKII (11533-1-AP, Proteintech), GAD67 (PA5-21397, Invitrogen), c-Fos (OB-PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523-1-AP, Proteintech). .. The sections were then incubated with fluorescence-conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5-μm-thick sections were stained using the TUNEL kit (A112, Vazyme).

    Incubation:

    Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress-Induced Depression in Male Mice.
    Article Snippet: For paraffin sections, the brains were embedded in paraffin (Thermo, USA), and then serially sectioned into 5-μm-thick transverse slices. .. For immunofluorescence analysis, the 30-μm-thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA-1 (GB12105, Servicebio), CaMKII (11533-1-AP, Proteintech), GAD67 (PA5-21397, Invitrogen), c-Fos (OB-PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523-1-AP, Proteintech). .. The sections were then incubated with fluorescence-conjugated secondary antibodies at room temperature for 4 h. All sections were stained with DAPI for 10 min. For cell death detection, the 5-μm-thick sections were stained using the TUNEL kit (A112, Vazyme).

    Article Title: Mechanism of Qufeng Huoxue decoction in treating paclitaxel-induced peripheral neuropathy based on network pharmacology and molecular docking.
    Article Snippet: Background and purpose: Chemotherapy-induced peripheral neuropathy (CIPN) is a common adverse reaction in cancer patients, affecting 30–50% of patients' quality of life.. Qufeng Huoxue Decoction (QHD), has been used for many years as an adjunctive treatment for CIPN in China.. However, the underlying mechanism of the role of Qufeng Huoxue Decoction in PIPN remains elusive.

    Article Title: 3D-printed biphasic calcium phosphate reinforced bioactive hydrogel scaffold for osteoporosis defect repair
    Article Snippet: The repair of osteoporotic bone defects faces dual challenges of abnormally elevated reactive oxygen species (ROS) levels in the microenvironment and an imbalance in osteoblast-osteoclast homeostasis.. There is an urgent need to develop bioactive materials that can regulate the microenvironment to promote bone regeneration.. In this study, a multifunctional composite scaffold reinforced with biphasic calcium phosphate (BCP) and combined with methacrylated silk fibroin (SilMA) hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) and tannic acid (TA), designated TB@SM-BCP, was constructed using 3D printing technology.

    Article Title: Apoptotic cell overload drives multinucleated giant cell formation after neoadjuvant chemotherapy in colorectal cancer: molecular mechanisms and tumor-suppressive effects
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). .. After blocking, the membranes were incubated overnight at 4 °C with primary antibodies against AKT1/2/3 (#T55561, Abmart, Shanghai, China), phospho-AKT (#T40067, Abmart, Shanghai, China), PI3K (#T40115, Abmart, Shanghai, China), phospho-PI3K (#T40116, Abmart, Shanghai, China), TYROBP (#R383147, ZENBIO, Chengdu, China), ERK1/2 (#I04101864, Wanleibio, Shenyang, China), phospho-ERK1/2 (#I06281512, Wanleibio, Shenyang, China), p38 (#T55600, Abmart, Shanghai, China), phospho-p38 (#310091, ZENBIO, Chengdu, China), JNK (#R22866, ZENBIO, Chengdu, China), phospho-JNK (Thr183) (#381100, ZENBIO, Chengdu, China), DCSTAMP (#A14630, ABclonal, Wuhan, China), c-FOS (#66590-1-Ig, Proteintech, Wuhan, China), and β-actin (#20536-1-AP, Proteintech, Wuhan, China). .. Subsequently, membranes were incubated with the corresponding HRP-conjugated goat anti-rabbit IgG or HRP-conjugated goat anti-mouse IgG secondary antibodies (Proteintech, Wuhan, China).

    Labeling:

    Article Title: Self-Assembled Rg3/Naringenin Nanoparticles for Targeted Brain Delivery: A Promising Therapeutic Approach for Early Alzheimer’s Disease
    Article Snippet: The ROS detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China) and the JC-1 detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). .. Primary antibody against ZO-1, Claudin-5, Occludin, p-ERK1/2, ERK1/2, c-Fos, Aβ and tau were all purchased from Proteintech Group, Wuhan, China and we labeled the horseradish peroxidase of the 2nd antibody as (PI Proteintech Group, Wuhan, China). ..

    Article Title: Self-Assembled Rg3/Naringenin Nanoparticles for Targeted Brain Delivery: A Promising Therapeutic Approach for Early Alzheimer’s Disease
    Article Snippet: The ROS detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China) and the JC-1 detection kit was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). .. Primary antibody against ZO-1, Claudin-5, Occludin, p-ERK1/2, ERK1/2, c-Fos, Aβ and tau were all purchased from Proteintech Group, Wuhan, China and we labeled the horseradish peroxidase of the 2nd antibody as (PI Proteintech Group, Wuhan, China). ..

    Blocking Assay:

    Article Title: 3D-printed biphasic calcium phosphate reinforced bioactive hydrogel scaffold for osteoporosis defect repair
    Article Snippet: The repair of osteoporotic bone defects faces dual challenges of abnormally elevated reactive oxygen species (ROS) levels in the microenvironment and an imbalance in osteoblast-osteoclast homeostasis.. There is an urgent need to develop bioactive materials that can regulate the microenvironment to promote bone regeneration.. In this study, a multifunctional composite scaffold reinforced with biphasic calcium phosphate (BCP) and combined with methacrylated silk fibroin (SilMA) hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) and tannic acid (TA), designated TB@SM-BCP, was constructed using 3D printing technology.

    Article Title: Apoptotic cell overload drives multinucleated giant cell formation after neoadjuvant chemotherapy in colorectal cancer: molecular mechanisms and tumor-suppressive effects
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). .. After blocking, the membranes were incubated overnight at 4 °C with primary antibodies against AKT1/2/3 (#T55561, Abmart, Shanghai, China), phospho-AKT (#T40067, Abmart, Shanghai, China), PI3K (#T40115, Abmart, Shanghai, China), phospho-PI3K (#T40116, Abmart, Shanghai, China), TYROBP (#R383147, ZENBIO, Chengdu, China), ERK1/2 (#I04101864, Wanleibio, Shenyang, China), phospho-ERK1/2 (#I06281512, Wanleibio, Shenyang, China), p38 (#T55600, Abmart, Shanghai, China), phospho-p38 (#310091, ZENBIO, Chengdu, China), JNK (#R22866, ZENBIO, Chengdu, China), phospho-JNK (Thr183) (#381100, ZENBIO, Chengdu, China), DCSTAMP (#A14630, ABclonal, Wuhan, China), c-FOS (#66590-1-Ig, Proteintech, Wuhan, China), and β-actin (#20536-1-AP, Proteintech, Wuhan, China). .. Subsequently, membranes were incubated with the corresponding HRP-conjugated goat anti-rabbit IgG or HRP-conjugated goat anti-mouse IgG secondary antibodies (Proteintech, Wuhan, China).

    Control:

    Article Title: 3D-printed biphasic calcium phosphate reinforced bioactive hydrogel scaffold for osteoporosis defect repair
    Article Snippet: The repair of osteoporotic bone defects faces dual challenges of abnormally elevated reactive oxygen species (ROS) levels in the microenvironment and an imbalance in osteoblast-osteoclast homeostasis.. There is an urgent need to develop bioactive materials that can regulate the microenvironment to promote bone regeneration.. In this study, a multifunctional composite scaffold reinforced with biphasic calcium phosphate (BCP) and combined with methacrylated silk fibroin (SilMA) hydrogel loaded with bone marrow mesenchymal stem cells (BMSCs) and tannic acid (TA), designated TB@SM-BCP, was constructed using 3D printing technology.



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    <t>ASIC3‐expressing</t> nociceptors are activated in the DRG and contribute to incision‐induced postoperative pain. (A–C) <t>Representative</t> <t>c‐Fos</t> immunostaining images and quantification in WT DRG sections on postoperative day 1. Scale bar = 20 µm, 20 × objective, zoom = 1.97 ×. Unpaired t tests; n = 4 mice/group, 3–4 sections/mouse. (D–F) Western blot analysis of ASIC3 expression in WT mouse DRG and spinal dorsal horn (SDH) on postoperative day 1. Unpaired t tests; n = 5 mice/group. (G,H) ASIC3 protein levels in the DRG of littermate WT mice and Trpv1 ; Cnr1 fl/fl mice on day 1 post‐incision. Unpaired t tests; n = 5 mice/group. (I) i.pl. injection of the selective ASIC3 blocker APETx2 on postoperative day 1 robustly reverses mechanical allodynia in WT mice. (J) PWT following i.pl. injection of the ASIC3 blocker APETx2 in naïve Trpv1 ; Cnr1 fl/fl mice. Two‐way ANOVA with Sidak's test for multiple comparisons; * p < 0.05, ** p < 0.01. n = 6–11 mice/group; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle.
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    ( A ) Schematic of histamine injection and experimental timeline. ( B ) Increased scratching bouts induced by intradermal injection of histamine in mice. **** P < 0.0001, Student’s t test, n = 5 to 6 mice. ( C and D ) Histamine-induced itch <t>increased</t> <t>c-fos</t> expression in multiple brain regions. (C) Representative images and (D) quantification of c-fos cells per mm 2 in saline and histamine-treated mice. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, Student’s t test, n = 4 to 7 mice. All data are shown as mean ± SEM. PFC, prefrontal cortex; CLA, claustrum; PV, paraventricular nucleus; PBN, parabrachial nucleus.
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    ( A ) Schematic of histamine injection and experimental timeline. ( B ) Increased scratching bouts induced by intradermal injection of histamine in mice. **** P < 0.0001, Student’s t test, n = 5 to 6 mice. ( C and D ) Histamine-induced itch <t>increased</t> <t>c-fos</t> expression in multiple brain regions. (C) Representative images and (D) quantification of c-fos cells per mm 2 in saline and histamine-treated mice. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, Student’s t test, n = 4 to 7 mice. All data are shown as mean ± SEM. PFC, prefrontal cortex; CLA, claustrum; PV, paraventricular nucleus; PBN, parabrachial nucleus.
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    ( A ) Schematic of histamine injection and experimental timeline. ( B ) Increased scratching bouts induced by intradermal injection of histamine in mice. **** P < 0.0001, Student’s t test, n = 5 to 6 mice. ( C and D ) Histamine-induced itch <t>increased</t> <t>c-fos</t> expression in multiple brain regions. (C) Representative images and (D) quantification of c-fos cells per mm 2 in saline and histamine-treated mice. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, Student’s t test, n = 4 to 7 mice. All data are shown as mean ± SEM. PFC, prefrontal cortex; CLA, claustrum; PV, paraventricular nucleus; PBN, parabrachial nucleus.
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    Image Search Results


    ASIC3‐expressing nociceptors are activated in the DRG and contribute to incision‐induced postoperative pain. (A–C) Representative c‐Fos immunostaining images and quantification in WT DRG sections on postoperative day 1. Scale bar = 20 µm, 20 × objective, zoom = 1.97 ×. Unpaired t tests; n = 4 mice/group, 3–4 sections/mouse. (D–F) Western blot analysis of ASIC3 expression in WT mouse DRG and spinal dorsal horn (SDH) on postoperative day 1. Unpaired t tests; n = 5 mice/group. (G,H) ASIC3 protein levels in the DRG of littermate WT mice and Trpv1 ; Cnr1 fl/fl mice on day 1 post‐incision. Unpaired t tests; n = 5 mice/group. (I) i.pl. injection of the selective ASIC3 blocker APETx2 on postoperative day 1 robustly reverses mechanical allodynia in WT mice. (J) PWT following i.pl. injection of the ASIC3 blocker APETx2 in naïve Trpv1 ; Cnr1 fl/fl mice. Two‐way ANOVA with Sidak's test for multiple comparisons; * p < 0.05, ** p < 0.01. n = 6–11 mice/group; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle.

    Journal: Advanced Science

    Article Title: Cannabinoid CB1 Receptor in Nociceptors Mediates Postoperative Analgesia via ASIC3 Inhibition

    doi: 10.1002/advs.77925

    Figure Lengend Snippet: ASIC3‐expressing nociceptors are activated in the DRG and contribute to incision‐induced postoperative pain. (A–C) Representative c‐Fos immunostaining images and quantification in WT DRG sections on postoperative day 1. Scale bar = 20 µm, 20 × objective, zoom = 1.97 ×. Unpaired t tests; n = 4 mice/group, 3–4 sections/mouse. (D–F) Western blot analysis of ASIC3 expression in WT mouse DRG and spinal dorsal horn (SDH) on postoperative day 1. Unpaired t tests; n = 5 mice/group. (G,H) ASIC3 protein levels in the DRG of littermate WT mice and Trpv1 ; Cnr1 fl/fl mice on day 1 post‐incision. Unpaired t tests; n = 5 mice/group. (I) i.pl. injection of the selective ASIC3 blocker APETx2 on postoperative day 1 robustly reverses mechanical allodynia in WT mice. (J) PWT following i.pl. injection of the ASIC3 blocker APETx2 in naïve Trpv1 ; Cnr1 fl/fl mice. Two‐way ANOVA with Sidak's test for multiple comparisons; * p < 0.05, ** p < 0.01. n = 6–11 mice/group; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle.

    Article Snippet: The sections were blocked for 1 h in 5% donkey serum (SL050, Solarbio) with 0.1% Triton X‐100 and incubated overnight at 4°C with primary antibodies against ASIC3 (ASC‐018, Alomone), c‐Fos (226017, SYSY), TRPV1 (ab6166, Abcam), CGRP (ab47027, Abcam), IB4 (L2895, Sigma), NF200 (N0142, Sigma), and pERK 1/2 (9101S, Cell Signaling Technology) at 4°C overnight.

    Techniques: Expressing, Immunostaining, Western Blot, Analysis, Injection

    ( A ) Schematic of histamine injection and experimental timeline. ( B ) Increased scratching bouts induced by intradermal injection of histamine in mice. **** P < 0.0001, Student’s t test, n = 5 to 6 mice. ( C and D ) Histamine-induced itch increased c-fos expression in multiple brain regions. (C) Representative images and (D) quantification of c-fos cells per mm 2 in saline and histamine-treated mice. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, Student’s t test, n = 4 to 7 mice. All data are shown as mean ± SEM. PFC, prefrontal cortex; CLA, claustrum; PV, paraventricular nucleus; PBN, parabrachial nucleus.

    Journal: Science Advances

    Article Title: Kappa opioids suppress histaminergic itch by inhibiting mediodorsal thalamus-projecting claustrum glutamatergic neurons

    doi: 10.1126/sciadv.ady4282

    Figure Lengend Snippet: ( A ) Schematic of histamine injection and experimental timeline. ( B ) Increased scratching bouts induced by intradermal injection of histamine in mice. **** P < 0.0001, Student’s t test, n = 5 to 6 mice. ( C and D ) Histamine-induced itch increased c-fos expression in multiple brain regions. (C) Representative images and (D) quantification of c-fos cells per mm 2 in saline and histamine-treated mice. Scale bar, 100 μm. * P < 0.05, ** P < 0.01, Student’s t test, n = 4 to 7 mice. All data are shown as mean ± SEM. PFC, prefrontal cortex; CLA, claustrum; PV, paraventricular nucleus; PBN, parabrachial nucleus.

    Article Snippet: C-fos antibody (rabbit anti c-fos) (226003, RRID: AB_2231974) was purchased from Synaptic Systems Company.

    Techniques: Injection, Expressing, Saline

    ( A and B ) Histamine-induced c-fos expression within CLA colocalized with vGlut1 mRNA, but not with GAD65 mRNA. (A) Left: Representative images, including c-fos (white), vGlut1 (green) and DAPI (blue); Right: Quantitative analysis (per mm 2 ). Scale bar, 50 μm. n = 3 mice. (B) Left: Representative images of the CLA, including c-fos (white), GAD65 (red) and DAPI (blue); Right: Quantitative analysis (per mm 2 ). Scale bar, 50 μm. n = 3 mice. ( C ) Schematic diagram and experimental procedure. ( D ) Representative image. Scale bar, 200 μm. ( E to H ) Representative traces of Ca 2+ signals of CLA glutamatergic neurons. (E) Synchronization of Ca 2+ signals with markers of scratching behavior. (F) Representative traces of Ca 2+ signals (red line) during scratching occur (−10 s − +10 s). n = 4 mice. (G) Heatmaps across animals aligned to the beginning of scratching. (H) Quantification of the area under the curve (AUC, −10–0 s, 0 s–10 s) of average Δ F/F . ** P < 0.01. Student’s t test. n = 4 mice. ( I ) Schematic diagram and experimental procedure. ( J and M ) Representative images of c-fos cells among CLA glutamatergic neurons. Scale bar, 50 μm, 20 μm. (J) The inhibitory effects of the hM4Di. (M) The excitatory effects of the hM3Dq. ( K and N ) Quantification of c-fos and mCherry co-labeled cells. (K) ** P < 0.01. Student’s t test, n = 3 mice. (N) *** P < 0.001. Student’s t test, n = 3 mice. ( L ) Chemogenetic inhibition of CLA glutamatergic neurons decreased histamine-induced scratching behavior. * P < 0.05. Student’s t test, n = 15 to 16 mice. ( O ) Chemogenetic activation of CLA glutamatergic neurons increased low-dose histamine-induced scratching behavior. * P < 0.05. Student’s t test, n = 8 to 9 mice. All data are shown as mean ± SEM.

    Journal: Science Advances

    Article Title: Kappa opioids suppress histaminergic itch by inhibiting mediodorsal thalamus-projecting claustrum glutamatergic neurons

    doi: 10.1126/sciadv.ady4282

    Figure Lengend Snippet: ( A and B ) Histamine-induced c-fos expression within CLA colocalized with vGlut1 mRNA, but not with GAD65 mRNA. (A) Left: Representative images, including c-fos (white), vGlut1 (green) and DAPI (blue); Right: Quantitative analysis (per mm 2 ). Scale bar, 50 μm. n = 3 mice. (B) Left: Representative images of the CLA, including c-fos (white), GAD65 (red) and DAPI (blue); Right: Quantitative analysis (per mm 2 ). Scale bar, 50 μm. n = 3 mice. ( C ) Schematic diagram and experimental procedure. ( D ) Representative image. Scale bar, 200 μm. ( E to H ) Representative traces of Ca 2+ signals of CLA glutamatergic neurons. (E) Synchronization of Ca 2+ signals with markers of scratching behavior. (F) Representative traces of Ca 2+ signals (red line) during scratching occur (−10 s − +10 s). n = 4 mice. (G) Heatmaps across animals aligned to the beginning of scratching. (H) Quantification of the area under the curve (AUC, −10–0 s, 0 s–10 s) of average Δ F/F . ** P < 0.01. Student’s t test. n = 4 mice. ( I ) Schematic diagram and experimental procedure. ( J and M ) Representative images of c-fos cells among CLA glutamatergic neurons. Scale bar, 50 μm, 20 μm. (J) The inhibitory effects of the hM4Di. (M) The excitatory effects of the hM3Dq. ( K and N ) Quantification of c-fos and mCherry co-labeled cells. (K) ** P < 0.01. Student’s t test, n = 3 mice. (N) *** P < 0.001. Student’s t test, n = 3 mice. ( L ) Chemogenetic inhibition of CLA glutamatergic neurons decreased histamine-induced scratching behavior. * P < 0.05. Student’s t test, n = 15 to 16 mice. ( O ) Chemogenetic activation of CLA glutamatergic neurons increased low-dose histamine-induced scratching behavior. * P < 0.05. Student’s t test, n = 8 to 9 mice. All data are shown as mean ± SEM.

    Article Snippet: C-fos antibody (rabbit anti c-fos) (226003, RRID: AB_2231974) was purchased from Synaptic Systems Company.

    Techniques: Expressing, Labeling, Inhibition, Activation Assay

    ( A and B ) Schematic diagram of experimental procedure. ( C ) Systemic injection of Nalfurafine and U50488H decreased histamine-induced scratching behavior. ** P < 0.01, **** P < 0.0001. Student’s t test, n = 5 to 6 mice. ( D and E ) Nalfurafine and U50488H decreased histamine-induced c-fos expression in CLA. (D) Representative images and (E) quantification of c-fos cells in CLA. Scale bar, 50 μm. * P < 0.05, ** P < 0.01, One-way ANOVA, n = 5 mice. ( F and I ) Schematic diagram of experimental procedure. ( G ) Microinjection of Nalfurafine and U50488H in the CLA decreased histamine-induced scratching behavior. ** P < 0.01, **** P < 0.0001. Student’s t test, n = 6 to 10 mice. ( H ) Schematic representation of Nalfurafine and U50488H microinjection sites in the CLA. ( J ) Microinjection of nor-BNI into the CLA blocked the antipruritic effects of systemically administered Nalfurafine and U50488H. *** P < 0.001, **** P < 0.0001, One-way ANOVA, n = 8 to 12 mice. ( K ) Schematic representation of nor-BNI microinjection sites in the CLA. All data are shown as mean ± SEM.

    Journal: Science Advances

    Article Title: Kappa opioids suppress histaminergic itch by inhibiting mediodorsal thalamus-projecting claustrum glutamatergic neurons

    doi: 10.1126/sciadv.ady4282

    Figure Lengend Snippet: ( A and B ) Schematic diagram of experimental procedure. ( C ) Systemic injection of Nalfurafine and U50488H decreased histamine-induced scratching behavior. ** P < 0.01, **** P < 0.0001. Student’s t test, n = 5 to 6 mice. ( D and E ) Nalfurafine and U50488H decreased histamine-induced c-fos expression in CLA. (D) Representative images and (E) quantification of c-fos cells in CLA. Scale bar, 50 μm. * P < 0.05, ** P < 0.01, One-way ANOVA, n = 5 mice. ( F and I ) Schematic diagram of experimental procedure. ( G ) Microinjection of Nalfurafine and U50488H in the CLA decreased histamine-induced scratching behavior. ** P < 0.01, **** P < 0.0001. Student’s t test, n = 6 to 10 mice. ( H ) Schematic representation of Nalfurafine and U50488H microinjection sites in the CLA. ( J ) Microinjection of nor-BNI into the CLA blocked the antipruritic effects of systemically administered Nalfurafine and U50488H. *** P < 0.001, **** P < 0.0001, One-way ANOVA, n = 8 to 12 mice. ( K ) Schematic representation of nor-BNI microinjection sites in the CLA. All data are shown as mean ± SEM.

    Article Snippet: C-fos antibody (rabbit anti c-fos) (226003, RRID: AB_2231974) was purchased from Synaptic Systems Company.

    Techniques: Injection, Expressing, Microinjection

    ( A ) Anterograde viral tracing from CLA to MD: schematic and representative fluorescence images. ( B ) Schematic and representative fluorescence images of retrograde viral tracing from CLA to MD. Scale bar, 100 μm. ( C to E ) Histamine-induced itch increased c-fos expression in the MD. (C) Schematic diagram of experimental procedure. (D) Representative images and (E) quantification of c-fos cells. Scale bar, 200 μm. * P < 0.05. Student’s t test, n = 8 mice. ( F to I ) MD glutamatergic neurons exhibit significant activation. (F) Schematic diagram and representative image and experimental procedure. Scale bar, 100 μm. (G to I) Representative traces of Ca 2+ signals of MD glutamatergic neurons. (G) Representative traces of Ca 2+ signals (blue line) during scratching occur (−10 s − +10 s). n = 3 mice. (H) Heatmaps across animals aligned to the beginning of scratching. (I) Quantification of the AUC (−10–0 s, 0 s–10 s) of average Δ F/F . *** P < 0.001. Student’s t test, n = 3 mice. ( J to M ) Axon terminals of CLA Glu neurons projecting to the MD exhibit significant activation during histamine-induced scratching behavior. (J) Schematic diagram and representative image and experimental procedure. Scale bar, 100 μm. (K) Representative traces of Ca 2+ signals (brown line) during scratching occur (−10 s − +10 s). n = 3 mice. (L) Heatmaps across animals aligned to the beginning of scratching. (M) Quantification of the AUC (−10–0 s, 0 s–10 s) of average Δ F/F . *** P < 0.001. Student’s t test, n = 3 mice. All data are shown as mean ± SEM.

    Journal: Science Advances

    Article Title: Kappa opioids suppress histaminergic itch by inhibiting mediodorsal thalamus-projecting claustrum glutamatergic neurons

    doi: 10.1126/sciadv.ady4282

    Figure Lengend Snippet: ( A ) Anterograde viral tracing from CLA to MD: schematic and representative fluorescence images. ( B ) Schematic and representative fluorescence images of retrograde viral tracing from CLA to MD. Scale bar, 100 μm. ( C to E ) Histamine-induced itch increased c-fos expression in the MD. (C) Schematic diagram of experimental procedure. (D) Representative images and (E) quantification of c-fos cells. Scale bar, 200 μm. * P < 0.05. Student’s t test, n = 8 mice. ( F to I ) MD glutamatergic neurons exhibit significant activation. (F) Schematic diagram and representative image and experimental procedure. Scale bar, 100 μm. (G to I) Representative traces of Ca 2+ signals of MD glutamatergic neurons. (G) Representative traces of Ca 2+ signals (blue line) during scratching occur (−10 s − +10 s). n = 3 mice. (H) Heatmaps across animals aligned to the beginning of scratching. (I) Quantification of the AUC (−10–0 s, 0 s–10 s) of average Δ F/F . *** P < 0.001. Student’s t test, n = 3 mice. ( J to M ) Axon terminals of CLA Glu neurons projecting to the MD exhibit significant activation during histamine-induced scratching behavior. (J) Schematic diagram and representative image and experimental procedure. Scale bar, 100 μm. (K) Representative traces of Ca 2+ signals (brown line) during scratching occur (−10 s − +10 s). n = 3 mice. (L) Heatmaps across animals aligned to the beginning of scratching. (M) Quantification of the AUC (−10–0 s, 0 s–10 s) of average Δ F/F . *** P < 0.001. Student’s t test, n = 3 mice. All data are shown as mean ± SEM.

    Article Snippet: C-fos antibody (rabbit anti c-fos) (226003, RRID: AB_2231974) was purchased from Synaptic Systems Company.

    Techniques: Fluorescence, Expressing, Activation Assay